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KMID : 1189220100370020099
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Korean Journal of Reproductive Medicine
2010 Volume.37 No. 2 p.99 ~ p.113
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Very Small Putative Stem Cells Detected in Human Endometrium
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Choi Jong-Ryeol
Joo Jong-Kil
Jun Eun-Sook
Ko Gyoung-Rae
Lee Hong-Gu
Lee Kyu-Sup
Kim Won-Gyu
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Abstract
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IObjective: It has been recently reported that very small stem cells with pluripotency are detected in murine and human. The purposes of this study are to confirm whether very small putative stem cells (VSPSCs), which have the proper characteristics of stem cells as well as the expression of stem cell markers, are detected in human endometrium.
Methods: The endometrial cells of 5 women, which were obtained by endometrial biopsy, were cultured for 2 weeks and were confirmed for the expressions of alkaline phosphatase, OCT-4 and CXCR4 by immunochemistry. Subsequently VSPSCs were separated by percoll density gradient method and were cultured. Also VSPSCs and their derived cells were confirmed for the expressions of OCT-4 and CXCR4.
Results: The colonies, which is composed with VSPSCs less than 3 μm and the 5~15 μm sized hyperchromatic round cells, were detected in the endometrium of all of women and showed the strong expressions of alkaline phosphatase, OCT-4 and CXCR4. In culture after the separation of VSPSCs by percoll, these cells showed the morphological and functional characteristics of stem cells; self-renewal, colony formation, embryoid body-like formation and differential plasticity. VSPSCs formed gradually the 5~15 μm sized hyperchromatic round cells and the 10~20 μm sized sphere-shaped cells by cell-to-cell aggregation or cell fusion. Then these cells differentiated the various cells including fibroblast-like cells, nerve-like cells and endothelium-like cells. VSPSCs and their derived cells often showed the strong expressions of OCT-4 and CXCR4.
Conclusion: VSPSCs less than 3 μm and their derived cells are detected in human endometrium and these cells have the proper characteristics of stem cells and the expressions of stem cell markers as alkaline phosphatase, OCT-4 and CXCR4.
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KEYWORD
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Endometrium, Stem cells, Alkaline phosphatase, OCT-4, CXCR4
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